Surveillance of Inducible Clindamycin Resistance of Staphylococcus aureus in Hyderabad.

Abstract

Objective: To evaluate and analyze the inducible and constitutive clindamycin resistance of Staphylococcus aureus from various clinical samples (i.e. blood, wound, nose, and urine in Hyderabad.

Methodology: Staphylococcus aureus isolates from various clinical specimens were included in this study. The sensitivity profiling against test antibiotics and determination of Inducible clindamycin resistance was performed by traditional Kirbeybaur disc diffusion and D-test respectively.

Results: The percentage of erythromycin resistant strains was 37% (n=56), of these ~43% (n=24) displayed constitutive clindamycin resistance while ~57% (n=32) were sensitive to clindamycin. Approximately two-third, 63% (n=20) of Clindamycin-
Erythromycin-Discordant strains displayed iMLSB phenotype. The percentage of iMLSB phenotype in Staphylococcus aureus
recovered from male and female was 64% (n=16) and 57.1% (n=4) respectively. OR was 1.33 [CI (95%) = 0.24-7.34].
Statistically non-significant differences (p-value >0.05) for iMLSB phenotype between genders were seen. iMLSB phenotype was maximum in case of blood isolates (87.5%) followed by wound isolates (70%). The highest percentage of iMLSB phenotype was seen in the age group of 51-60 years. Pearson’s co-relation co-efficient and p-value were determined to be (r=0.639, p<0.05), suggesting a significantly positive co-relation between various age groups and iMLSB phenotype.

Conclusion: The iMLSB phenotype of Staphylococcus aureus is independent of gender and significantly positively co-related with increasing age of the patients.
Key words: S. aureus, Clinical specimen, iMLSB, cMLSB, Clindamycin, Hyderabad.