Comparative Efficacy and Antimicrobial Activity of Three Different Disinfectants and Standard Disinfectant against Diverse Pathogens.

Abstract

Objective: To determine the efficacy and antimicrobial activity of three different brands of disinfectant against pathogens.
Methodology:  The present study was conducted at department of microbiology, Herbion Pharmaceutical (Pvt.) Limited Karachi, Pakistan. Our standard disinfectant contains , whereas comparative disinfectants Brand A containing cetrimide and chlorhexidine gluconate, Brand B lemon disinfectant containing Didecyl dimethyl ammonium chloride and branched, ethoxylated 4-nonylphenol and Brand C which contains Alkyl (60%C14, 25%C12, 15%C16) dimethylbenzyl ammonium chloride . The zone of inhibition or Antimicrobial susceptibility pattern of these disinfectants was noted against Staphylococcus aureus (ATCC-6538),
Escherichia coli (ATCC-8739), Pseudomonas aeruginosa (ATCC- 9027), Bacillus subtilis (ATCC-6633), Aspergillus niger (ATCC-16404), Candida albicans (ATCC-10231).
Results: The different pattern of millimeter zone of inhibition is detected when compared directly in concentrated form and with the different dilution; 1:10 and 1:100ml of disinfectant against various pathogens.
Conclusion: It was concluded that Chloroxylenol (Standard Disinfectant) is more favorable and frequently used in pharmaceutical
industries and considered to be more reliable as compare to other disinfectant. The surface or inanimate object are more secure and germ free after cleaning with standard disinfectant. The second-best choice in cleaning after Chloroxylenol is Brand B lemon disinfectant containing Didecyl dimethyl ammonium chloride and branched, ethoxylated 4-Nonylphenol.

Key Words: Disinfectant, Pathogen, Antimicrobial activity, Bacteria, Molds, Yeasts.